The Eben Samuel Johnson Collection is primarily composed of the Bishop' s papers but also includes records from his immediate family as well. The collection does not end with the death of the Bishop. It continues with correspondence between Sarah Tilsley Johnson and her children, especially with Samuel Darlow. The collection reflects the close interconnected relationships within the Johnson family. These were individuals whose characteristics were unique to themselves yet found a commonality within their love for each other and the church.

There are a total of nine series which cover a variety of subjects and record types. Included are correspondence, a journal, various writings, clippings, biographical material, and a racoon coat. Topics embrace David Livingstone, various church organizations, mission work, cultural anthropology, educational institutions, industry, cannibalism, governments, sermons, world events, etc. Much of Johnson's autographs are written in Pitman shorthand.

The collection contains photographs about missionary work done in Africa, mainly in Angola. There are several photographs from England, Germany, Holland and the Canary Islands. There are several event programs, one each from Ohio, Virginia and Africa and newspaper clippings concerning missionaries and Mrs. Glidden's marriage.